《植物生理学报》 2017, 53(12): 2181-2188

褪黑素对葡萄果实成熟及乙烯和ABA含量的影响

许丽丽, 岳倩宇, 卞凤娥, 翟衡, 姚玉新^*

山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点实验室, 山东泰安271018

通信作者：姚玉新；E-mail: yaoyx@sdau.edu.cn

摘　要：

褪黑素不仅是一种抗氧化剂, 而且能作为激素调控植物生长和发育。本文主要研究了外源褪黑素处理对葡萄果实成熟的影响, 并测定了其对成熟调控因子乙烯和ABA的影响。结果表明, 外源褪黑素处理能极显著提高果实内源褪黑素含量。褪黑素处理提高了总花青苷和可溶性固形物含量, 降低了可滴定酸含量, 表明褪黑素促进了葡萄果实成熟。RNA-Seq分析表明褪黑素处理显著诱导了乙烯生化合成和信号传导关键基因的表达水平, 包括2个ACS、2个ACO和10个ERF。进一步分析表明褪黑素处理提高了转色期葡萄果实中乙烯前体物质ACC含量和乙烯释放速率。此外, 研究还发现褪黑素处理6h后显著下调了ABA合成基因NCED1, 上调了ABA降解基因CYP707A1的表达, 但对ABA含量未产生显著性影响。总之, 本研究表明褪黑素促进了葡萄果实的成熟, 乙烯很可能在此过程中起关键作用。

关键词：褪黑素; 乙烯; ABA; 成熟; RNA-Seq; 葡萄

收稿：2017-06-13   修定：2017-11-17

资助：山东省自然科学基金(ZR2015CM014)、农业产业技术体系(CARS-30-ZT-06)和山东省“双一流”奖补资金(SYL2017YSTD10)。

Effects of melatonin treatment on grape berry ripening and contents of ethylene and ABA

XU Li-Li, YUE Qian-Yu, BIAN Feng-E, ZHAI Heng, YAO Yu-Xin^*

College of Horticultural Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, Taian, Shandong 271018, China

Corresponding author: YAO Yu-Xin; E-mail: yaoyx@sdau.edu.cn

Abstract:

Melatonin acts not only an antioxidant, but also a hormone that regulates plant growth and development. In this paper, the effects of external melatonin treatment on the ripening of grape (Vitis vinifera) fruit were studied, and its effects on mature regulatory factors of ethylene and ABA were determined. It was found that the content of endogenous melatonin of grape was significantly improved in the treatment. Melatonin treatment increased the contents of anthocyanins and TSS and decreased the titratable acidity, indicating the accelerated berry ripening. RNA-Seq analysis showed that melatonin treatment significantly induced the expression of the key genes related to ethylene biosynthesis and signaling, including two ACSs, two ACOs and ten ERFs. Further research indicated that melatonin treatment enhanced the content of ethylene biosynthesis substrate ACC and ethylene release rate in grape berries. Besides, it was also found that melatonin treatment significantly decreased the expression levels of NCED1 (ABA biosynthesis) and enhanced CYP707A1 (ABA degradation), respectively. In contrast, melatonin did not affect the ABA content of grape berries in a statistically significant manner. Therefore, it was indicated that melatonin promoted berry ripening and ethylene most likely played a key role in this process.

Key words: melatonin; ethylene; ABA; berry ripening; RNA-Seq; grape (Vitis vinifera)